ChIP-Atlas: SRX20178002

Antigen

Cell type Class: Neural
Cell type: Microglia
MeSH Description: The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling.

library_name: GSM7273512
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: The cells/nuclei were immobilized with Con-A beads and incubated with an antibody overnight. The immunocomplexes were further incubated with protein A-fused micrococcal nuclease (pA-MNase), then digested for 30 min in the presence of 3mM Ca2++ (Ref: PMID: 28079019). If needed, the ChIPped DNA was de-crosslinked and purified by phase extraction. The DNA was filtered with 0.5x AMPure beads to remove unwanted large fragments. Purified ChIPed DNA was then processed to construct a library using SMARTer® ThruPLEX DNA-Seq Kit (TAKARA Bio USA, CA) as the manufacturer's protocol except PCR cycles to avoid amplifying long fragments (13 cycles, 10s for extension).